Lab Anim Res.  2010 Dec;26(4):407-413. 10.5625/lar.2010.26.4.407.

Antioxidative and Antidiarrheal Effects of Persimmon Extracts

• Park D
• Lee SH
• Bae DK
• Cho YJ
• Kim TK
• Yang YH
• Yang G
• Kwon SC
• Lee DI
• Joo SS
• Kim YB

• KMID: 2114710
• DOI: http://doi.org/10.5625/lar.2010.26.4.407

Abstract

Since oxidative stresses are involved in gastroenteritis and diarrhea, we investigated antioxidative and antidiarrheal activities of persimmon flesh extract (PFE) and persimmon calyx extract (PCE) in vitro and in vivo, respectively. PCE significantly scavenged 1,1-diphenyl-2-picrylhydrazyl hydrate and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) from 500 microg/mL, although PFE was ineffective. In addition, PFE and PCE exhibited strong nitric oxide-scavenging effects from 1 microg/mL, in which PCE was superior to ascorbic acid (50 microM). Furthermore, PFE and PCE significantly inhibited FeCl3-induced lipid peroxidation as well as Cu2+/H2O2-induced protein oxidation from 10 microg/mL. In vivo charcoal-propulsion assay, in contrast to a negligible effect of PFE, treatment with PCE (160-500 mg/kg) markedly inhibited intestinal motility. The results indicate that extracts of persimmon, especially PCE, possess antioxidative, antiinflammatory and antidiarrheal activities. Therefore, it is suggested that persimmon extracts could be used for the relief of gastroenteritis and diarrhea.

Keyword

Persimmon; antioxidative activity; antidiarrheal activity; gastrointestinal motility

MeSH Terms

Ascorbic Acid
Biphenyl Compounds
Diarrhea
Diospyros
Gastroenteritis
Gastrointestinal Motility
Lipid Peroxidation
Oxidative Stress
Picrates
Ascorbic Acid
Biphenyl Compounds
Picrates

Figure

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  Figure 1. 1,1-Diphenyl-2-picrylhydrazyl radical-scavenging activities of persimmon flesh extract (PFE) and persimmon calyx extract (PCE). Asc: ascorbic acid (50 µM). ∗Significantly different from vehicle control (100%) (P<0.05).

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  Figure 2. 2,2'-Azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS)-scavenging activities of persimmon flesh extract (PFE) and persimmon calyx extract (PCE). Asc: ascorbic acid (50 µM). ∗Significantly different from vehicle control (100%) (P<0.05).

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  Figure 3. Nitric oxide-scavenging activities [% of control (100%)] of persimmon flesh extract (PFE) and persimmon calyx extract (PCE). Asc: ascorbic acid (50 µM).

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  Figure 4. Inhibitory effects of persimmon flesh extract (PFE) and persimmon calyx extract (PCE) on FeCl3-induced lipid peroxidation. BHT: butylated hydroxytoluene (10 µM). ∗Significantly different from vehicle control (100%) (P<0.05).

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  Figure 5. Inhibitory effects of persimmon flesh extract (PFE) and persimmon calyx extract (PCE) on Cu2+/H2O2-induced protein [bovine serum albumin (BSA)] oxidation. Asc: ascorbic acid (50 µM).

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  Figure 6. Inhibitory effects of persimmon flesh extract (PFE) and persimmon calyx extract (PCE) on carbachol (Carb, 0.5 mg/kg)-induced gastrointestinal motility (charcoal propulsion). Asc: ascorbic acid (50 µM). ∗Significantly different from Carb alone (P<0.05).

Reference

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