Korean J Physiol Pharmacol.  2003 Oct;7(5):275-278.

A Simple and Accurate Method for Determining Antioxidative Activity

• Bae JW
• Lee MH

Abstract

Antioxidative activity is an important factor in inhibiting oxidative stress. The usual methods for determining antioxidative activity are time-consuming and cumbersome. They are also indirect processes that use biological material such as brain or liver microsome. This study therefore proposed a new method. Redoxpotential was determined using galvanic cell with or without the addition of various antioxidants or herbal extracts in zinc sulfate solution. The result was compared with the results from the TBA method and the peroxide value from sodium thiosulfate titration. All methods showed significant and dose-dependent enhancement of antioxidative activity by adding ascorbic acid, quercetin, ginseng, or gingko biloba extract. The result of redox potential using galvanic cell showed the smallest standard deviation and took the shortest time among the three methods. Therefore, the antioxidative potential of chemical substances and herbal extracts can be determined simply, directly and accurately in a short period of time using galvanic cell.

Keyword

Antioxidative activity; Redoxpotential; Galvanic cell; Thiobarbituric acid; Sodium thiosulfate

MeSH Terms

Antioxidants
Ascorbic Acid
Brain
Ginkgo biloba
Microsomes, Liver
Oxidation-Reduction
Oxidative Stress
Panax
Quercetin
Sodium
Zinc Sulfate
Antioxidants
Ascorbic Acid
Quercetin
Sodium
Zinc Sulfate