J Korean Ophthalmol Soc.  2009 Mar;50(3):424-428. 10.3341/jkos.2009.50.3.424.

The Effect of Dorzolamide-Timolol and Latanoprost on Redox Cycle in Aqueous Humor of Rabbit

• Park SW
• Lee SH
• Mun GH

• KMID: 2111254
• DOI: http://doi.org/10.3341/jkos.2009.50.3.424

Abstract

PURPOSE: To evaluate the effect of dorzolamide-timolol and latanoprost on redox cycle of superoxide dismutase (SOD) and ascorbic acid in the aqueous humor of the rabbit.
METHODS
Group 1 (20 eyes of 10 rabbits) was instilled with dorzolamide-timolol in the right eye and Group 2 (20 eyes of 10 rabbits) was instilled with latanoprost in the same manner. Four weeks after instillation, SOD activity and ascorbic acid concentration were analyzed.
RESULTS
In Group 1, SOD activity and ascorbic acid concentration of the right eye and left eye were 15.7% and 19.3% (p=0.27), 2.7 pM and 2.6 pM (p=0.47), respectively. In Group 2, SOD activity and ascorbic acid concentration of the right and left eye were 22.3% and 27.6% (p=0.14), 2.3 pM and 2.1 pM (p=0.89), respectively.
CONCLUSIONS
There was no significant difference of SOD activity and ascorbic acid concentration between both eyes in Group 1 and 2.

Keyword

Ascorbic acid; Dorzolamide-timolol; Latanoprost; Superoxide dismutase

MeSH Terms

Aqueous Humor
Ascorbic Acid
Eye
Oxidation-Reduction
Prostaglandins F, Synthetic
Superoxide Dismutase
Ascorbic Acid
Prostaglandins F, Synthetic
Superoxide Dismutase

Figure

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  Figure 1. Standard curve for ascorbic acid assay.

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  Figure 2. (A, B) Superoxide dismutase (SOD) activities in the aqueous humor of both eyes. (A) After instillation of Dorzolamide-Timolol (black bar) and (B) Latanoprost (black bar) in the right eye, respectively. There were no significant differences between both eyes.

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  Figure 3. (A, B) Ascorbic acid concentration in the aqueous humor of both eyes. (A) After instillation of Dorzolamide-Timolol (black bar) and (B) Latano-prost (black bar) in the right eye, respectively. There were no significant differences between both eyes.

Reference

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