The Effect of Retinoic Acid on Experimentally-Induced Proliferative Vitreoretinopathy in the Rabbit

Hyun, Duk Hun; Yang, Han Nam

Abstract

The proliferative vitreoretinopathy was a complication followed by operation of rhegmatogenous retinal detachment. It was the mot, comon cause of a failure of retinal detachment surgery. It was characterized by the growth of cellular fibrous membrance in detached both retinal side and vitreous and also developed by giant retinal dialysis, posterior segmental trauma, excessive cryotherapy, endophthalmitis, retianl vascular disease. In order to prevent and treat of proliferative vitreoretinopathy, various methods of operation and drugs have been researched. We executed the experiment using the rabbit to observe the effect of retinoic acid that is known by inhibition of migration and proliferation of retinal pigment epithelial cell and fibroblast in vitro. With 121 eyes of rabbit, we induced the proliferative vitreoretinopathy by injecting of human retinal pigment epithelial cell, human fibroblast, and rabbit fibroblast into eyeball of rabbits. The extent of proliferative vitreoretinopathy was compaired by injecting those cells with the group that was injected by retinoic acid and control. The result was that in those cell groups, the extent of proliferative vitreoretinopathy was significantly higher in the rabbit fibroblast group than the other two groups(P<0.05). And in the groups that were injected retinoic acid, when subconjuctivaly injected(0.3mg/0.3ml), proliferative vitreoretinopathy was effectively suppressed and when intravitrealy injected (0.05mg/0.1ml), vitreoretinopathy was more increased than the control group. This result was probably caused by high concentration of retinoic acid in vitreous and further evaluation with various concentration of retinoic acid is needed. In conclusion, we recommend a rabbit fibroblast and subconjunctival injection of retinoic acid for the study on the suppressive effect of proliferative vitreoretinopathy.