J Korean Ophthalmol Soc.  1995 Jan;36(1):67-74.

Demonstration of Virus in Retinal Necrosis following Intravitreal Inoculation of Herpes Simplex Virus-1

• Ko MK
• Lee HY
• Choe JK

Abstract

This study was performed to observe the retinal changes in rabbits by intravitreal inoculation of HSV-1. The solution of HSV-1 virus(Kos strain) was inoculated into the vitreous cavity in 5 eyes. All the eyes were checked with a slit lamp and an indirect ophthalmoscope. Two eyes that showed the retinal lesion were enucleated for the histopathologic examination. The focal infiltration of inflammatory cells was marked around the retinal vessels in one eye and there was the diffuse infiltration of plasma cells and lymphocytes in the choroid and the retina in the other eye. The pattern of proliferation and migration of retinal pigment epithelial cells was observed at the outer retina. The virus-infected nuclei were evident in the photoreceptor cells and ganglion cells. The viral particles and intranuclear inclusion were prominent in the deformed nuclei and free-floating viral particle was shown at the extracellular space of the necrotic retina. These results suggest that the intravitreal inoculation of HSV-1 might induce retinal necrosis. The inflammatory reactions was initiated at the vitreoretinal interface and perivascular area. Virus might be propagated through axons or infected cell from free-flating virus.

Keyword

Herpes simplex virus; intravitreal inoculation; retinal necrosis

MeSH Terms

Axons
Choroid
Epithelial Cells
Extracellular Space
Ganglion Cysts
Herpes Simplex*
Herpesvirus 1, Human
Intranuclear Inclusion Bodies
Lymphocytes
Necrosis*
Ophthalmoscopes
Photoreceptor Cells
Plasma Cells
Rabbits
Retina
Retinal Vessels
Retinaldehyde*
Simplexvirus
Virion
Retinaldehyde