J Korean Soc Spine Surg.  1998 May;5(1):25-32.

The Effects of TGF-beta1 & TGF-beta2 on the Proliferation of the Lumbar Facet Joint Capsule in Tissue Culture

• Lee KY
• Yoo YH
• Cho KW

Abstract

STUDY DESIGN: This study was undertaken to document the effect of TGF-beta 1 and TGF-beta2 on the proliferation of lumbar facet joint capsule.
OBJECTIVES
To study the effect of TGF-beta1 and TGF-beta2 on the proliferation of lumbar facet joint capsule and their proliferation mechanism. SUMMARY OF LITERATURE REVIEW: TGF-beta1 and TGF-beta proliferated mesenchymal tissue . This proliferatine mechanism was involved of PLC-gamma- 1 and tyrosine kinase in signalling. MATERIAL AND METHODS: Three lumbar facet joint capsule was cultured in DMEM-20 media. Its proliferatine and inhibited effect was studied under the metabolic inhibitors and trasnsforming growth factors .
RESULTS
TGF-beta1, and TGF-beta2, increased cell proliferation dependent on time and dosage. Both of TGF-beta1, and TGF-beta 2, specific antisense oligonucleotide blocked tile autoc.me effect of growth factor, PLC-gamma-1 specific antisense oligonucleotide inhibited the effect of TGF-beta 1, and TGF-beta2, Genistin inhibited the effect of TGF-beta 1, and TGF-beta2, in time and dosage dependent manner.
CONCLUSION
The cell proliferation of lumbar facet joint capsule was involved in PLC- gamma-1 and tyrosine kinase in signalling.

MeSH Terms

Cell Proliferation
Intercellular Signaling Peptides and Proteins
Protein-Tyrosine Kinases
Transforming Growth Factor beta
Transforming Growth Factor beta1*
Transforming Growth Factor beta2*
Zygapophyseal Joint*
Intercellular Signaling Peptides and Proteins
Protein-Tyrosine Kinases
Transforming Growth Factor beta
Transforming Growth Factor beta1
Transforming Growth Factor beta2