J Korean Surg Soc.  2001 Sep;61(3):305-311.

Microsatellite Alterations of Chromosome 9p, 13q, 16q in Hepatocellular Carcinoma

• Cho SJ
• Kim NR
• Min YK
• Joh YG
• Cho MY
• Suh SO
• Yeom BW
• Won NH

Abstract

PURPOSE: Hepatocellular carcinoma (HCC) patients are asymptomatic and the tumor remains in an unresectable state until the tumor progresses. Recently much efforts for elucidation of the early hepatocarcinogenesis have been made, and for this purpose it is very crucial to investigate the genetic abnormalities. We evaluated microsatellite alterations of five markers from chromosome 9, 13, 16 and investigated the relationships with the clinicopathological parameters in HCC.
METHODS
The microsatellite alteration analysis was performed using polymerase chain reaction with five polymorphic microsatellite markers (D9S171, D9S1747, D13S156, D16S419, D16S3106) in 40 surgically resected HCCs and their respective non-tumorous counterparts.
RESULTS
D9S171, D9S1747, D13S156, D16S419, D16S3106 abnormalities were detected in 20.0%, 14.3%, 50.0%, 32.4% and 22.6%, respectively. Loss of heterozygosity (LOH) of D9S171 correlated well with higher tumor histologic grade and LOH of D13S156, D16S419 and D16S3106 correlated well with increased tumor size. Microsatellite instability (MSI) was found in two markers, D13S156, D16S419.
CONCLUSION
As a result, we concluded that alterations in microsatellites of various chromosomes may contribute to the hepatocarcinogenesis and tumor progression. Especially LOH of chromosome 13 and 16 are considered to correlate with tumor progression.

Keyword

Hepatocellular carcinoma; Microsatellite; Loss of heterozygosity

MeSH Terms

Carcinoma, Hepatocellular*
Chromosomes, Human, Pair 13
Chromosomes, Human, Pair 9
Humans
Loss of Heterozygosity
Microsatellite Instability
Microsatellite Repeats*
Polymerase Chain Reaction