Korean J Dermatol.
1994 Aug;32(4):669-674.
Immunohistochemical Study of Acantholytic Cells of the Squamous Cell Carcinoma of the Skin
Abstract
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BACKGROUND: Acantholysis can be seen occasionally in the cutanous squamous cell carcinoma(SCC) as a result of degenerative changes of neoplastic cells.
OBJECTIVE
This study was done to investigate the keratin attern and a wide range of immunohistochemical features of acantholytic cells of cutaneous SCC.
METHODS
Seventeen cases of SCC showed acantholytic cells histoloieally and formalin-fixed, paraf-finembedded biopsy specimens from them were stained by ABC(avidin-biotin-peroxidase complex) staining. Fourteen biopsy specimens from 14 cases of SCC were staincd with 3 monoclonal anti-keratin antibodies(CAM 5.2, MAK-6, and 34bE12) and 17 biopsy spec:mcns from 17 cases of SCC were stained with antibodies agairist CEA(carcinoembryonic antigen), vitamin, S-100 protein, Factor VIII-R Ag, LCA(leukocyte common antigen), and lysozyme. RESULT & CONCLUSION: Acantholytic cells of 14 cases of SCC showed consistently negative staining with CAM 5.2. The acatholytic cells showed a wide range of reactivity with MAK-6 from negative to moderately strong positivity and with 34pE12 from negative to strong positivity. A few acantholytic cells of 6 cases of SCC showed weakly positive staining with anti-CEA antibody, but acantholytic cells of all 17 cases showed consistently negative staining wit,h the other antibodies.
