Korean J Fertil Steril.
2000 Jun;27(2):201-208.
Effect of Fertilization Promoting Peptide on Kinematic Parameters, Capacitaion and Acrosome Reaction in Human Spermatozoa
Abstract
OBJECTIVE
This study has been carried out to evaluation the effect of fertilization promoting peptide (FPP) on the kinematic parameters, capacitation and acrosome reaction of the frozen-thawed human spermatozoa.
METHODS
After FPP treatment, we examined kinematic parameters, capacitation and acrosome reaction, using the methods of computer-aided sperm analysis (CASA) and chlortetracycline (CTC) fluorescence analysis.
RESULTS
We have obtained the evidence that FPP can promote the capacitation and inhibit the spontaneous acrosome reaction of frozen-thawed human spermatozoa in vitro. Fpp (25~100 nM) induced a significant increase in the proportion of B-pattern capacitated spermatozoa, and a significant decrease in the proportion of F-pattern uncapacitated ones without significant stimulation of acrosomal exocytosis. In the kinematic parameters treatment, FPP treated groups maintained higher LIN, BCF and STR than those of control. The VAP, VSL, VCL and ALH were not different. Therefore it is suggested that FPP in human seminal plasma may play a positive role in promoting human sperm function.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Effect of Fertilization Promoting Peptide (FPP) on the Acrosome Status of Cryopreserved Human Sperm
- Ca2+-ATPase Role in the Capacitation and Acrosome Reaction Assessed by a Chlortetracycline Fluorescence Assay
- Efficacy of Acrosome Reaction after Ionophore Challenge (ARIC) Test in Evaluation of Fertilization Capacity of Human Spermatozoa
- Spermatozoa Characteristics of Streptozotocin-induced Diabetic Wistar Rat: Acrosome Reaction and Spermatozoa Concentration
- Observation of the Incidence of Acrosome Reaction in Human Spermatozoa Treated with Mibefradil as a T-type Ca2+i Channels Inhibitor
