Korean J Gastroenterol.  1999 Jan;33(1):11-19.

Role of Glutathione and Protective Effect of Cysteamine in the Ammonia Induced Gastric Mucosal injury -Related with the Mechanism of Helicobacter pylori Associated Gastric Diseases ??

• Roe IH
• Lee MI
• Hwang HC
• Kim HY
• Chin YJ
• Lim CY
• Song IH
• Kim JW
• Yang MR
• Cho CH
• Lee JH

Abstract

BACKGROUND/AIMS: The mechanism of gastric mucosal injury by ammonia, one of the toxic products of Helicobacter pylori, is still unclear. We investigated the role and protective effect of glutathione in the ammonia-induced gastric mucosal injury.
METHODS
The male Sprague Dawley rats were sacrified after intragastric administration of 1 ml of 1% ammonia with pretreating intragastric cysteamine (Cys-am group), or intraperitoneal buthionine sulfoximine (BSO-am group), or without any pretreatment (Cont-am group). Additional groups of rats were pretreated with equivalent cysteamine (Cys group) or eqivalent BSO (BSO group) and without any treatment (Cont group) and then, sacrified. The gastric mucosal glutathione (GSH) and malondialdehyde (MDA) were measured and the severity of gastric mucosal damage was evaluated microscopically.
RESULTS
Gastric mucosa GSH was considerably increased in the Cys group and the Cys-am group (p<0.001 and p<0.0001 v Cont group, respectively). Gastric MDA was increased significantly in the Cont-am group or in the BSO-am group. However, the MDA levels in the Cys-am group did not markedly change comparedto that of the control. The pathologic results of the Cys-am group showed minimal injuries, but extensive hemorrhage and erosions were observed in the Cont-am or the BSO-am group.
CONCLUSIONS
GSH depletion was an important factor in the pathogenesis of ammonia-induced gastric mucosal injury. This results suggest that the pathogenetic role of H. pylori is related with gastric diseases and cysteamine have a strong protective effect against those gastric damages.

Keyword

Ammonia; Gastric mucosal injury; Glutathione; Helicobacter pylori; Cysteamine

MeSH Terms

Ammonia*
Animals
Buthionine Sulfoximine
Cysteamine*
Gastric Mucosa
Glutathione*
Helicobacter pylori*
Helicobacter*
Hemorrhage
Humans
Male
Malondialdehyde
Rats
Rats, Sprague-Dawley
Stomach Diseases*
Ammonia
Buthionine Sulfoximine
Cysteamine
Glutathione
Malondialdehyde