Korean J Gynecol Oncol Colposc.  1997 Dec;8(4):381-387.

Study for MIB-1 Immunostaining and DNA Ploidy in Endometrial Carcinoma

• Lim KT
• Kim TJ
• Chung HW
• Lee GH
• Park JT
• Park IS
• Shim JU
• Kim HS
• Kim YJ
• Chun YK
• Hong SR
• Kim HS

Abstract

OBJECTIVE: The monoclonal antibody MIB-1 reacts with the same antigen as Ki67 antigen giving an estimate of growth fraction. The authors investigated whether MIB-1 staining, using semiquantative analysis and DNA ploidy study, could be a prognostic indicator of recurrence in endometrial carcinoma.
METHODS
The tumors from 45 consecutive patients receiving primary surgical therapy for endometrial carcinoma were evaluated with MIB-1 monoclonal antibody and DNA ploidy status. Growth fraction was quantified by counting stained nucleus in HPF. DNA ploidy study was evaluated by flow cytometry. The patients were followed for 5 years and their charts were reviwed to determine recurrence, stage,and grade.
RESULTS
MIB-1 staing was significantly elevated in histologic high grade (grade II and III) as opposed to low grade(grade I) carcinoma (P=0.038). Aneuploidy was significantly increased incidence in advaned stage (stage III and IV) as opposed to early stage(stage I and II) (P=0.017). MIB-1 staing and aneuploidy were associated with increased incidence of recurrence within 5 years of diagnosis, but not statistically significance.
CONCLUSION
In this series of 45 patients with endometrial carcinoma. MIB-1 monoclonal antibody staining and aneuploidy to be a prognostic indicators of recurrence.

MeSH Terms

Aneuploidy
Diagnosis
DNA*
Endometrial Neoplasms*
Female
Flow Cytometry
Humans
Incidence
Ploidies*
Recurrence
DNA