Korean J Infect Dis.  1997 Mar;29(2):139-146.

Production of Monoclonal Antibody to Chlamydia Pneumoniae

• Choi TY
• Kang JO
• Kim DU
• Ahn JY
• Choi HS

Abstract

BACKGROUND: Chlamydia pneumoniae is a recently recognized species consisting of the strains commonly referred to as TWAR. These strains are associated with acute respiratory infections in humans, especially atypical pneumonia. So we tried to make a monoclonal antibody to Chlamydia pneumoniae.
METHODS
C. pneumoniae were adapted to grow in HeLa-229 cells. The organisms were harvested and purified in a linear gradient of renograffin. BALB/c mice (female, 10weeks) were intravenously immunized with purified C. pneumoniae(TW-183).The spleen cells and SP 2/0 myeloma cells were fused with 40% polyethylene glycol (Mol.Wt.:1,450). Antibodies against C. pneumoniae were screened by an enzyme- linked immunosorbent assay (ELISA). The proteins of purified chlamydial elementary bodies were separated by discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and Western blots were performed with these monoclonal antibodies.
RESULTS
Two monoclonal antibodies (HYMD10, HYMG12) reacted specifically with C. pneumoniae, as measured by an ELISA and indirect immunofluorecent stain. One of the monoclonal antibody (HYMD10) reacted with 75- and 39-KDa proteins in Western blot. The other monoclonal antibody (HYMG12) reacted with 98- and 39-KDa proteins of C. pneumoniae.
CONCLUSIONS
These species-specific monoclonal antibodies (HYMD10, HYMG12) to C. pneumoniae could be used for diagnosis of C. pneumoniae infections.

Keyword

Chlamydia pneumoniae; Monoclonal antibody; Western blot

MeSH Terms

Animals
Antibodies
Antibodies, Monoclonal
Blotting, Western
Chlamydia*
Chlamydophila pneumoniae*
Diagnosis
Diatrizoate Meglumine
Electrophoresis
Enzyme-Linked Immunosorbent Assay
Humans
Mice
Pneumonia
Polyethylene Glycols
Respiratory Tract Infections
Sodium
Spleen
Antibodies
Antibodies, Monoclonal
Diatrizoate Meglumine
Polyethylene Glycols
Sodium