Korean J Med.  1997 Jun;52(6):743-753.

A Study for Detection of HBV by Sandwitch in situ Hybridization in Chronic Liver Disease

• Lee SH
• Kim HS
• Park CS
• Kim SJ

Abstract

OBJECTIVES
In situ hybridization(ISH) is widely applied as an effective method to detect the positive signal in the preservation of histological architecture with high specificity. The purpose of this study is to evaulate the clinical availability of SISH(sand-witch in situ hybridization) using HBsAg mRNA probe in biopsy specimen of patients with chronic liver disease.
METHODS
SISH was employed to detect the HBsAg mRNA & DNA in 127 cases of chronic liver diseases and the results were compared with those obtained by immunohistochemistry and enzyme immunoassay methods,
RESULTS
The HBsAg mRNA & DNA by SISH and the HBsAg by immmunohistochemistry were detected in 94 cases(92.2%) and 86 cases(84.3%) of 102 HBsAg seropositive cases, respectively. The detection of HBsAg mRNA & DNA by SISH was identified in 8 cases of 25 HBsAg seronegative cases of chronic liver disease, and the HBsAg by immunohistochemistry in 1 case. The detection rates of HBsAg mRNA & DNA by SISH were 78.3% in chronic persistent hepatitis, 81.7% in chronic active hepatitis, 87.5% in liver cirrhosis, and 70.6% in hepatocellular carcinoma. The positive rates of HRsAg by immunohistochemistry were 56.5% in chronic persistent hepatitis, 71.8% in chronic active hepatitis, 87.5% in liver cirrhosis, and 52.9% in hepatocellular carcinoma. The detection of HBsAg mRNA & DNA by SISH is more sensitive than that of HBsAg by immunohistochemistry.
CONCLUSION
SISH using HBsAg mHNA probe is a useful and sensitive method to detect HBV in chronic liver disease, and it is more sensitive than immunohistochemistry.

Keyword

HRsAg mRNA probe; Sandwitch in situ hybridization SISH; Immunohistochemistry IHC; Chronic liver disease

MeSH Terms

Biopsy
Carcinoma, Hepatocellular
DNA
Hepatitis B Surface Antigens
Hepatitis, Chronic
Humans
Immunoenzyme Techniques
Immunohistochemistry
In Situ Hybridization*
Liver Cirrhosis
Liver Diseases*
Liver*
RNA, Messenger
Sensitivity and Specificity
DNA
Hepatitis B Surface Antigens
RNA, Messenger