Modulation of endometrial Interleukin-1 Receptor Type 1

Lee, B Y; Yoon, H H; Kim, S B

Abstract

The Interleukin-1 system has been suggested as an important modulator for implanta-tion process, although its clear mechanism of action has not been elucidated. In this study we investigate the response of interleukin-1 receptor type1 in cultured endometrial cells by various embryonic products known to be involved in successful pregnancy outcome, includ-ing IL-1beta, platelet activating factor (PAF) and human chorionic gonadotropin (hCG). Human endometrial tissue was separated to glandular epitherial cells and stromal cells. After 6 days culture of confluent monolayer in the serum containg media with progesterone, endometrial cell layers were further cultured with 6ml media each containing IL-1beta (10 IU/ml), PAF (200 nM) or hCG (10 IU/ml). After 24 to 48 hr of culture endometrial cell layers were scraped and IL-1RtI expression was determined by Northern blot analysis. Endometrial expression of IL-1RtI messenger ribonucleic acid (mRNA) was increased until 24 hours and did not ch-anged until 48 hours. IL-1RtI mRNA expression of endometrial stromal cells cultured with IL-1beta and PAF were higher than that of control (P>0.05). HCG did not affect the endome-trial stromal IL-1RtI mRNA expression. These results suggest that IL-1 system plays a role in embryo- endometrial communication.