Korean J Pediatr Hematol Oncol.
2001 Oct;8(2):323-330.
Identification of HLA-B*5001 in Korean Population
- Affiliations
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- 1Department of Pediatrics, St' Francisco Hospital, Seoul, Korea.
- 2Hemopoietic Stem Cell Bank, College of Medicine, The Catholic University of Korea, Seoul, Korea.
- 3Department of Microbiology and Immunology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Abstract
- PURPOSE: HLA (human leukocyte antigen)-class I genes are highly polymorphic, play many roles in organ and bone marrow transplantation. HLA-B is the most polymorphic class I locus with 414 alleles. HLA-class I typing, which is based on serologic method, has been used until recently. The development of molecular biological techniques make it possible to define the genotypes of HLA genes.
METHODS
Analyses of HLA-B genotyping on 1,000 UCB (Umbilical Cord Blood) samples which were considered to be HLA-B homozygote or blank were performed by ARMS-PCR (Amplification Refractory Mutation System-PCR) method and direct sequencing.
RESULTS
We could identify HLA-B*5001 which was known to be absent in Koreans.
CONCLUSION
It is strongly suggested that HLA-B homozygote should be confirmed to the DNA level especially in cases of donor selection for the unrelated bone marrow transplantation.
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