Korean J Perinatol.  2009 Dec;20(4):305-314.

The Association between the Development of Preeclampsia and Methylenetetrahydrofolate Reductase, Angiotensinogen, Vascular Endothelial Growth Factor Single Nucleotide Polymorphism Genotype Combinations

• Park HS
• Jun JK

Abstract

PURPOSE: Preeclampsia is one of the most frequent complications in pregnancy. Because many different phenotypes are seen with this disease, more than one gene is thought to be involved in the pathogenesis of preeclampsia. This study was conducted to investigate if there exists any genotype combination of multiple single nucleotide polymorphism(SNP)s which are frequently found in preeclampsia patients.
METHODS
One hundred and sixty two preeclampsia patients and 199 normotensive pregnant women were included. Severity was assigned and the patients were re-classified as early-onset (less than 31 weeks) and late-onset (31 weeks or beyond) disease. Genotype was measured with polymerase chain reaction-restriction fragment length polymorphism for Methylenetetrahydrofolate reductase (MTHFR) C677T, Angiotensinogen (AGT) M235T, and Vascular endothelial growth factor (VEGF) C936T. Case-control study for each SNP was done and the frequencies of genotype combinations were compared. ANOVA, t-test, chi-square test, Fisher's exact test and logistic regression analysis were used for statistical analysis. A P value of <0.05 was considered significant.
RESULTS
Genotypes of MTHFR C677T polymorphism showed significant difference between late onset preeclampsia and control (CC+CT/TT, OR: 1.82, P<0.05) but AGT M235T and VEGF C936T polymorphism did not show statistically significant difference between any SNP combinations. Of the possible 27 genotype combinations, only 20 genotype combinations were found and there was no statistical difference in frequency of genotype combinations between case and control group.
CONCLUSION
MTHFR C677T polymorphism might be associated with the development of preeclampsia, However combination of MTHFR C677T, AGT M235T and VEGF C936T polymorphisms were not associated with the development of preeclampsia.

Keyword

Preeclampsia; Single nucleotide polymorphism (SNP); Genotype combination

MeSH Terms

Angiotensinogen
Case-Control Studies
Female
Genotype
Humans
Logistic Models
Methylenetetrahydrofolate Reductase (NADPH2)
Phenotype
Polymorphism, Single Nucleotide
Pre-Eclampsia
Pregnancy
Pregnant Women
Vascular Endothelial Growth Factor A
Angiotensinogen
Methylenetetrahydrofolate Reductase (NADPH2)
Vascular Endothelial Growth Factor A