J Korean Neurosurg Soc.  2011 Jun;49(6):323-328. 10.3340/jkns.2011.49.6.323.

Effect of Amniotic Membrane to Reduce Postlaminectomy Epidural Adhesion on a Rat Model

• Choi HJ
• Kim KB
• Kwon YM

• KMID: 2066828
• DOI: http://doi.org/10.3340/jkns.2011.49.6.323

Abstract

OBJECTIVE
Epidural fibrosis and adhesion are the main reasons for post-laminectomy sustained pain and functional disability. In this study, the authors investigate the effect of irradiated freeze-dried human amniotic membrane on reducing epidural adhesion after laminectomy on a rat model.
METHODS
A total of 20 rats were divided into two groups. The group A did not receive human amniotic membrane implantation after laminectomy and group B underwent human amniotic membrane implantation after laminectomy. Gross and microscopic findings were evaluated and compared at postoperative 1, 3 and 8 weeks.
RESULTS
The amount of scar tissue and tenacity were reduced grossly in group of rats with human amniotic membrane implantation (group B). On a microscopic evaluation, there were less inflammatory cell infiltration and fibroblast proliferation in group B.
CONCLUSION
This experimental study shows that implantation of irradiated freeze-dried human amniotic membrane reduce epidural fibrosis and adhesion after spinal laminectomy in a rat model.

Keyword

Human amniotic membrane; Failed back surgery syndrome; Epidural adhesion; Laminectomy

MeSH Terms

Amnion
Animals
Cicatrix
Failed Back Surgery Syndrome
Fibroblasts
Fibrosis
Humans
Laminectomy
Rats