J Korean Assoc Maxillofac Plast Reconstr Surg.  2010 Mar;32(2):118-125.

BONE METASTASIS MODEL OF ORAL SQUAMOUS CELL CARCINOMA

• Park YW
• Oh YJ
• Lee HS

Abstract

BACKGROUND AND PURPOSE
Bone metastases rarely occur in patients with oral squamous cell carcinoma (OSCC), so the molecular mechanisms of bone metastasis of OSCC remains unclear. Studies with animal models allow progresses in understanding the molecular events for bone metastasis and provide new targets for therapy. So we tried to establish a murine model for bone metastasis of oral squamous cell carcinoma.
MATERIALS AND METHODS
Human OSCC cells (KB cell line) were xenografted to nude mice via direct inoculation into the tibial marrow. Mice with tibial tumors were sacrificed once a week, until seven weeks after the injection of human tumor cells. Growth of tibial tumors were observed by histology. Expression of TGF-beta and CXCR-4 in bone OSCC (experimental) and subcutaneous tumor (control) was also evaluated by immunohistochemical staining.
RESULTS
Bone OSCC was successfully induced by intra-tibial injection of KB cells. Tumor mass was developed in the marrow tissues of tibia and finally invade the endosteum of tibia. Immunohistochemical staining showed higher expression of TGF-beta in bone tumors than in subcutaneous tumors.
CONCLUSION
A murine model of bone metastasis of OSCC was suggested that imitated the clinical findings of distant vascular metastasis. This bone tumor model should facilitate understanding of the molecular pathogenesis of OSCC bone metastasis, and aid in the developement of treatment strategies against OSCC bone metastasis.

Keyword

Oral squamous cell carcinoma; Bone metastasis; Animal model

MeSH Terms

Animals
Bone Marrow
Carcinoma, Squamous Cell
Humans
KB Cells
Mice
Mice, Nude
Models, Animal
Neoplasm Metastasis
Tibia
Transforming Growth Factor beta
Transplantation, Heterologous
Transforming Growth Factor beta