J Korean Acad Periodontol.  1997 Dec;27(4):949-961. 10.5051/jkape.1997.27.4.949.

A study of the effects of electric current on the mineralzation of the cultured calvaria bone cells

• Park JB
• Hur IS
• Lee HJ
• Choi YC

• KMID: 2057018
• DOI: http://doi.org/10.5051/jkape.1997.27.4.949

Abstract

To date, various clinical procedures have been used to restore periodontal apparatus destroyed by periodontal disease. And then, many experimental approaches have been proceeded to develop treatment methods to promote periodontal regeneration. Mechanical, chemical treatments to enhance the attachment of periodontal tissue cells as changing the physical properties of root surfaces, bone graft procedure, and treatments for guided tissue regeneration have been used for periodontal regeneration. However, recent studies have revealed that biologic factors such as growth factors promote biologic mechanism associated with periodontal regeneration. This study was done to enucleate how ELF stimulus affect the periodontal regeneration. We can have following conclusions from this experimental results. The influence of low frequency(ELF) electric stimulus (30HZ at 10micronA) known to promote bone formation in vivo, was evaluated for its ability to affect bone cell function in vitro. After 12 hour exposure of ELF stimulus at most appropriate densities (5x10(4) cells/cm2) to increase osteoblastic cells normally, rat calvarial cells were incubated for 60 hours were used in this study. We have found ELF stimulus suppress calvarial cell proliferation and the ability of protein synthesis, enhance the alkaline phosphatase activity significantly.

MeSH Terms

Alkaline Phosphatase
Animals
Biological Factors
Cell Proliferation
Guided Tissue Regeneration
Intercellular Signaling Peptides and Proteins
Osteoblasts
Osteogenesis
Periodontal Diseases
Rats
Regeneration
Skull*
Transplants
Alkaline Phosphatase
Biological Factors
Intercellular Signaling Peptides and Proteins