J Clin Pathol Qual Control.  1998 Dec;20(2):411-415.

The Measurement of Homocyst(e)ine by IMx Analyzer

• Kim HS
• Lee YK
• Lee KM
• Jung JY

Abstract

BACKGROUND: Hyperhomocyst(e)inemia is a risk factor for vasoocclusive diseases. High plasma homocyst(e)ine level can be usually reversed with vitamin supplements. Quantifying concentration of homocyst(e)ine helps identify patients who might benefit from the intervention. Current methods of measuring homocyst(e)ine are based on high performance liquid chromatography (HPLC). Recently, the IMx Homocysteine assay (Abbott Laboratories, IL, USA ) by fluorescence polarization immunoassay has been developed. We evaluated IMx Homocysteine assay.
METHODS
Between-day precisions of the IMx Homocysteine Controls (H: 25.0 micronmol/L, L:7.0 micronmol/L) for 10 days. Plasma homocyst(e)ine concentration was determined in 30 healthy subjects by the IMx Homocysteine assay. Correlation with HPLC was evaluated for 34 plasma samples from patients.
RESULTS
Between-day precisions (CVs) of IMx Homocysteine assay were 1.6% and 2.5% for Controls H and L, respectively. Plasma honocyst(e)ine levels from healthy subjects were 7.92+/-2.22 micronmol/L (mean+/-SD), in good agreement with reported values. The comparison study indicated good correlation between the IMx Homocysteine assay and HPLC, and correlation efficient (r) was 0.941 (P<0.01).
CONCLUSIONS
The results by the IMx Homocyst(e)ine assay show excellent precision and good correlation with those by HPLC. The IMx Homocysteine assay may be useful for quantifying homocyst(e)ine in clinical laboratories

MeSH Terms

Chromatography, High Pressure Liquid
Chromatography, Liquid
Fluorescence Polarization Immunoassay
Homocysteine
Humans
Plasma
Risk Factors
Vitamins
Homocysteine
Vitamins