J Clin Pathol Qual Control.
1998 Dec;20(2):407-410.
HLA-DR15 and HLA-DR16 Typing with Sequence-Specific Primer-Polymerase Chain Reaction
- Affiliations
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- 1Department of Clinical Pathology, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea.
Abstract
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BACKGROUND: AMPLICOR HLA DRB (Hoffmann-La Roche, Germany), which is being used for HLA-DR typing, is unable to discriminate HLA-DR15 from HLA-DR16, splits of HLA-DR2. Because HLA-DR15 and HLA-DR16 are frequent and distributed at a ratio of about 9:1 among Koreans, it is required to distinguish DR15 and DR16, especially for patients who are candidates for unrelated bone marrow transplantation.
METHODS
For 75 specimens typed as HLA-DR2 by AMPLICOR HLA DRB, we performed polymerase chain reaction (PCR) with two sets of sequence-specific primers (SSP) which are designed to be specific for DRB*15 and DRB*16, respectively.
RESULTS
All 75 specimens were easily confirmed to be DR15 or DR16, exclusively. 73 samples of HLA-DR15 were also shown to have 'DRB5*01' only, or 'DRB5*01 or DRB5*02', while another 2 samples of HLA-DR16 were shown to have 'DRB5*02' only by AMPLICOR HLA DRB.
CONCLUSIONS
HLA-DR15 and -DR16 were easily distinguished by SSP-PCR for specimens typed as DR2 by AMPLICOR HLA DRB. Even though information of DRB5 genotypes is very useful to discriminate HLA-DR15 from HLA-DR16, it is thought that this kind of additional typing will be necessary especially for the patients awaiting unrelated bone marrow transplantation.
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