J Clin Pathol Qual Control.  1998 Dec;20(2):401-405.

Laboratory Evaluation of the Coulter GEN.S System Five-Part Differential

• Park JY
• Lee KH
• Lee YK
• Cho HC

Abstract

BACKGROUND: The leukocyte differential count provides useful informations for the diagnosis of various diseases including hematologic disorders. Newly developed automated hematology analyzers of many manufacturers have been introduced in our country. We evaluated the performance of the Beckman-Coulter's the newest model, Coulter GEN.S system (GEN.S), on differential leukocyte counts.
METHODS
The leukocyte differentials of 285 patients' samples provided by the GEN.S were compared with those obtained from the manual reference method and the Technicon H*2 hematology analyzer (H*2). The reference values of the leukocyte differential for the GEN.S were calculated from the results of 104 heathy adults.
RESULTS
The correlation with the manual method was good for neutrophils, lymphocytes, eosinophils, and monocytes, and it was poor for basophils (r=0.956, 0.955, 0.943, 0.813, 0.415). When compared with the manual method, underestimation of lymphocytes and overestimation of neutrophils and monocytes were observed (P<0.001). The correlation with the H*2 was also good except for basophils. The reference mean values of the leukocyte differential for the GEN.S were 53.9% for neutrophils, 36.0% for lymphocytes, 7.1% for monocytes, 2.4% for eosinophils, and 0.5% for basophils.
CONCLUSIONS
The leukocyte differential of GEN.S showed good correlation with the manual counts, which would make it possible to report the rapid and reliable results. when combined with appropriate morphologic checking criteria based on suspect and definitive flags and it's own reference values.

MeSH Terms

Adult
Basophils
Diagnosis
Eosinophils
Hematology
Humans
Leukocyte Count
Leukocytes
Lymphocytes
Monocytes
Neutrophils
Reference Values