Gut Liver.  2013 Jan;7(1):30-34.

Detection of Helicobacter pylori in Gastric Aspirates Using a Monoclonal Antibody-Based Test

• Kim HD
• Kim DH
• Park H
• Kim WJ
• Ahn YS
• Lee YJ
• Park SM
• Seo ES
• Park C
• Kim YH
• Kim HR
• Joo YE
• Jung YD

Abstract

BACKGROUND/AIMS
The objective of this study was to evaluate a monoclonal antibody-based test to detect Helicobacter pylori-specific antigen in gastric aspirates from humans.
METHODS
Sixty-one volunteers were enrolled in the study. All of the subjects underwent a 13C-urea breath test (UBT) before esophagogastroduodenoscopy. Gastric aspirates were analyzed for pH and ammonia and used for polymerase chain reaction (PCR), culture, and monoclonal antibody-based detection of H. pylori. Multiple biopsies of the gastric antrum and body were obtained for a rapid urease test (RUT) and histological evaluation.
RESULTS
Thirty-six subjects were H. pylori-positive and 25 were H. pylori-negative according to the UBT results. Compared with the H. pylori-negative subjects, H. pylori-positive subjects had a higher pH (4.77+/-1.77 vs 3.49+/-1.30, p<0.05) and ammonia level (1,130.9+/-767.4 vs 184.2+/-126.3, p<0.0001). The sensitivities and specificities of the PCR test, RUT, culture test, and monoclonal antibody-based test were 100% and 72%, 89% and 100%, 47% and 100%, and 78% and 100%, respectively.
CONCLUSIONS
The monoclonal antibody-based test for diagnosing H. pylori infection in gastric aspirates has increased sensitivity compared with the culture test and specificity as high as that of the RUT. The test may be useful as an additive test for examining gastric aspirates.

Keyword

Helicobacter pylori; Gastric aspirate; Monoclonal antibody-based test

MeSH Terms

Ammonia
Biopsy
Breath Tests
Endoscopy, Digestive System
Helicobacter
Helicobacter pylori
Hydrogen-Ion Concentration
Polymerase Chain Reaction
Pyloric Antrum
Sensitivity and Specificity
Urease
Ammonia
Urease