Mycobiology.  2009 Dec;37(4):317-319. 10.4489/MYCO.2009.37.4.317.

Development of Specific Primer for Tricholoma matsutake

• Kim JH
• Han YH

• KMID: 2053037
• DOI: http://doi.org/10.4489/MYCO.2009.37.4.317

Abstract

In this study, in an effort to develop a method for the molecular detection of Tricholoma matsutake in Korea from other closely related Tricholomataceae, a species-specific PCR primer pair, TmF and TmR, was designed using nuclear ribosomal intertranscribed spacer (ITS) sequences. The DTmF and DTmR sequences were 5'-CCTGACGCCAATCTTTTCA-3' and 5'-GGAGAGCAGACTTGTGAGCA-3', respectively. The PCR primers reliably amplified only the ITS sequences of T. matsutake, and not those of other species used in this study.

Keyword

ITS; Tricholoma matsutake; Tricholomataceae

MeSH Terms

Korea
Polymerase Chain Reaction
Tricholoma