Mycobiology.  2007 Jun;35(2):54-61.

Proliferation of Tricholoma matsutake Mycelial Mats in Pine Forest Using Mass Liquid Inoculum

Affiliations
  • 1Department of Applied Biology, Kangwon National University Chuncheon 200-701, Korea. jmsung@kangwon.ac.kr
  • 2Mushtech, Keunhwa-Dong 798-3, Chunchoen 200-931, Korea.
  • 3Yangyang Agriculture Technology, Yangyang, Korea.
  • 4Department of Botany and Plant Pathology, Oregon State University, Corvallis, USA.

Abstract

Two isolates of Tricholoma matsutake T-008 and T-034, preserved in Entomopathogenic Fungal Culture Collection (EFCC) of Korea, were used in the present study. The isolates had 100% Bootstrap homology with Tricholoma matsutake U62964 and T. matsutake AB188557 and AF309538 preserved in Gene Bank of NCBI. Mycelial growth of T. matsutake was highest in TMM and MYA at 25degrees C. The highest dry wt. of mycelium was obtained after 65 days of culture, when 6 mycelial discs were inoculated in 100 ml of broth in 250 ml shaking flask. Mycelial mats were observed in clumped condition at the inoculation sites of pine forest after two weeks of inoculation. After 5 months of inoculation, mycelia mats were observed growing inside soil and walls of a few inoculation sites, while mycelial mats growth up to 5~8 cm were observed in the roots of pine tree after 6 months. The survival rate of the inoculum was about 40% of the total inoculation sites. The survival rate was found below 20% when the mycelium was inoculated in the summer. The reasons for low survival rates of the mycelium were mainly due to dry season and the soil-borne small animals such as earthworm and mole. After one year of inoculation, no external difference was observed between the artificially inoculated mycelia and the naturally existing mycelia of T. matsutake. The present study showed that fruiting bodies of T. matsutake could be produced by artificial inoculation under the appropriate environmental conditions.

Keyword

Liquid inoculum; Mycelial mats; Tricholoma matsutake; Phylogenetic analysis

MeSH Terms

Animals
Fruit
Korea
Mya
Mycelium
Oligochaeta
Pinus
Seasons
Soil
Survival Rate
Trees*
Tricholoma*
Soil
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