Mycobiology.  2009 Dec;37(4):247-250. 10.4489/MYCO.2009.37.4.247.

Mating Types and Optimum Culture Conditions for Sexual State Formation of Fusarium fujikuroi Isolates

• Choi HW
• Kim JM
• Hong SK
• Kim WG
• Chun SC
• Yu SH

• KMID: 2053023
• DOI: http://doi.org/10.4489/MYCO.2009.37.4.247

Abstract

Twenty-five isolates of Fusarium fujikuroi acquired from rice seeds and rice plants evidencing symptoms of Bakanae disease were evaluated to determine their mating types and characterize the formation of their sexual state. The mating types of the isolates were evaluated via multiplex PCR with the diagnostic primers of the mating-type (MAT) region: GFmat1a, GFmat1b, GFmat2c, and GFmat2d. Among the 25 isolates, 11 were identified as MAT-1 (male), and 14 as MAT-2 (female). Four MAT-1 isolates and three MAT-2 isolates were mated and cultured to evaluate the optimal culture conditions for the production of their sexual states. Among four tested media, 10% V8 juice agar proved optimal for the perithecial production of the isolates. The isolates also generated the largest numbers of perithecia when incubated at 23degrees C in alternating cycles of 12 hr fluorescent light and NUV fluorescent light and 12 hr darkness.

Keyword

Bakanae disease; Fusarium fujikuroi; Gibberella fujikuroi; Mating types; Sexual state formation

MeSH Terms

Agar
Darkness
Fusarium
Light
Multiplex Polymerase Chain Reaction
Seeds
Agar