Mycobiology.  2002 Sep;30(3):139-145. 10.4489/MYCO.2002.30.3.139.

Genotypic Identification of Fusarium subglutinans, F. proliferatum and F. verticillioides Strains Isolated from Maize in Austria

• Gherbawy Y
• Adler A
• Prillinger H

• KMID: 1612355
• DOI: http://doi.org/10.4489/MYCO.2002.30.3.139

Abstract

Gibberella fujikuroi is species complex. This species complex includes Fusarium tabacinum, F. moniliforme (= F. verticillioides), F. nygamai, F. proliferatum as well as F. subglutinans. Our objective was to develop a technique to differentiate between isolates of F. subglutinans, F. proliferatum and F. verticillioides. Thirty-two strains of F. subglutinans, six strains from F. verticillioides and five strains of F. Proliferatum isolated from maize in Austria were studied using random amplified polymorphic DNA (RAPD). F. subglutinans strains clustered very closely, with similarity ranging from 87~100%. On the other hand, all the amplification patterns of F. verticillioides were identical, as well as in the case of F. proliferatum. Our results indicated that these Fusaria species are distinct species and hence RAPD markers can be quick and reliable for differentiating them.

Keyword

Fusarium; F. moniliforme; Gibberella fujikuroi; RAPD-PCR; Phylogenetics

MeSH Terms

Austria*
DNA
Fusarium*
Gibberella
Hand
Random Amplified Polymorphic DNA Technique
Zea mays*
DNA