Mycobiology.  2009 Sep;37(3):218-224. 10.4489/MYCO.2009.37.3.218.

Production of Blastospore of Entomopathogenic Beauveria bassiana in a Submerged Batch Culture

• Pham TA
• Kim JJ
• Kim SG
• Kim K

• KMID: 2053017
• DOI: http://doi.org/10.4489/MYCO.2009.37.3.218

Abstract

The principal objective of this study was to determine the optimal liquid culture conditions in shake flasks for maximal sporulation of Beauveria bassiana. The optimal initial pH for the spore production of B. bassiana using Potato Dextrose Broth was 5.2. The screening in shake flasks of carbon and nitrogen sources resulted in the identification of an optimal medium based on 3% sucrose and 1% casamino acid, with a C : N ratio of 22 : 4. Using this medium, a production level of 5.65 x 107 spores per ml was obtained after 5 days of culture. Using 3% corn meal, 2% corn steep powder, and 2% rice bran, the maximum spore concentration of 8.54 x 108/ml was achieved 8 days after inoculation at 25degrees C in a rotary shaking incubator operated at 200 rpm. This represents a yield gain of approximately 2.89 times that of pre-optimization.

Keyword

Beauveria bassiana; Blastospore production; Liquid medium; Optimal culture conditions

MeSH Terms

Batch Cell Culture Techniques
Beauveria
Carbon
Glucose
Hydrogen-Ion Concentration
Incubators
Mass Screening
Meals
Nitrogen
Solanum tuberosum
Spores
Sucrose
Zea mays
Carbon
Glucose
Nitrogen
Sucrose