Mycobiology.
2001 Dec;29(4):179-182.
Cloning and Phylogenetic Analysis of Chitin Synthase Genes from Tricholoma matsutake
- Affiliations
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- 1Department of Microbiology, College of Natural Sciences, Kyungpook National University, 1370 Sangyuk-dong, Daegu 702-701, Korea. kimjg@knu.ac.kr
- 2Department of Food, Beverage and Culinary Arts, Taegu Technical College, Daegu 704-721, Korea.
- 3Korean Collection for Type Cultures, Genectic Resources Center, Korea Research Institute of Bioscience and Biotechnology, Daejon 305-600, Korea.
Abstract
- Chitin synthases(UDP-N-acetyl-D-glucosamine: chitin 4-beta-N-acetyl-D-glucosaminyl transferase, EC 2.4.1.16) catalyze the synthesis of chitin from UDP-N-acetyl-D-glucosamine. Two zymogenic type of chitin synthase gene(TmCHS1 and TmCHS2) were amplified and its nucleotide sequences were determined. By the amino acid comparison and UPGMA tree grouping, TmChs1 and TmChs2 were classified as class II and class IV chitin synthases respectively. The class II type TmChs1 was grouped with others of Agaricales ectomycorrhizal mushroom. Additionally the phylogenetic tree was well adapted to Hymenomycete previously classified by morphological and physiological characteristics.
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