Ann Surg Treat Res.  2015 Jul;89(1):17-22. 10.4174/astr.2015.89.1.17.

The effects of ventilation with high density oxygen on the strength of gastrointestinal anastomosis

• Eker T
• Genc V
• Sevim Y
• Cumaogullari O
• Ozcelik M
• Kocaay AF
• Ensari CO
• Pasaoglu O

• KMID: 2047709
• DOI: http://doi.org/10.4174/astr.2015.89.1.17

Abstract

PURPOSE
The aim of our study is to evaluate the effects of administration of perioperative supplemental oxygen on anastomoses.
METHODS
Forty male Wistar albino rats were used in the study and randomized into 4 groups. Ischemia-reperfusion models were built in groups 3 and 4. Jejunojejunostomy was performed in all rats and assigned to an oxygen/nitrous oxide mixture with a fraction of inspired oxygen of 30% in groups 1 and 3 and 80% in groups 2 and 4. The measurements of perianastomotic tissue oxygen pressure, bursting pressure, level of hydroxyproline were evaluated and compared in all groups.
RESULTS
The perianastomotic tissue oxygen pressures, bursting pressures and levels of hydroxyproline were identified as significantly high in groups 2 and 4, administered a fraction of inspired oxygen of 80%, compared to groups 1 and 3, administered a fraction of inspired oxygen of 30%.
CONCLUSION
Perioperative supplemental oxygen contributes positively to the anastomotic healing.

Keyword

Anastomosis; Wistar rats; Oxygen

MeSH Terms

Animals
Humans
Hydroxyproline
Male
Oxygen*
Rats
Rats, Wistar
Ventilation*
Hydroxyproline
Oxygen

Figure

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  Fig. 1 The median values of perianastomotic oxygen pressures for each group. We evaluated perianastomotic oxygen pressure for each group, and identified the values of group 2 significantly higher than group 1 (P < 0.001). Similarly, we found a significant high value of perianastomotic oxygen pressure for group 4 compared with group 3 (P < 0.001). However, there were no significant differences between groups 1 and 3 and also between groups 2 and 4 (P > 0.05).

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  Fig. 2 The median values of anastomotic bursting pressures for each group.

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  Fig. 3 The median values of tissue hydroxyproline levels for each group.

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