Korean J Obstet Gynecol.  2010 Dec;53(12):1100-1109. 10.5468/kjog.2010.53.12.1100.

The study of association between human papillomavirus and Chlamydia trachomatis infection in cervical intraepithelial neoplasia

• Yang JS
• Jeong YH
• Kim JS

• KMID: 2037511
• DOI: http://doi.org/10.5468/kjog.2010.53.12.1100

Abstract

OBJECTIVE
The aim of this study is to comparing the prevalence and correlationships between human papillomavirus (HPV) and Chlamydia trachomatis (CT) infection in cervical samples among women with abnormal cervical cytology.
METHODS
This study was included three hundred seventy four patients with a abnormal liquid-based cytology in Dankook University hospital. All of them underwent HPV DNA test and CT analysis with polymerase chain reaction. All patients also went through colposcopic directed cervical biopsies or Loop Electrosurgical Excision Procedure, conization. The histo-pathologic results were classified as normal, cervical intraepithelial neoplasia (CIN) 1, CIN 2, CIN 3 and carcinoma in situ (CIS).
RESULTS
Abnormal liquid-based cytology patients were pathologically proven to have CIN. Among 374 patients, the number of within normal limits (chronic cervicitis) and koilocytosis was 186 cases (49.7%), CIN 1, 64 cases (17.1%), CIN 2, 16 cases (4.3%) CIN 3, 55 cases (14.7%), and CIS, 53 cases (14.2%). HPV DNA positive patients were 235 cases and HPV DNA negative patients were 139 cases. The impact of CT infection seems not to interfere with the development or even the progression of CIN. Thirty one patients had positive infection of CT (8.3%) and 343 patients were negative infection of CT (91.7%). Both HPV and CT positive infected patients were 25 cases (6.7%) in abnormal cytologic women. The correlation between HPV and CT DNA positive among women with abnormal cytology was statistically significant. (P=0.022)
CONCLUSION
This study suggests that CT infection is associated with HPV infection, but the clinical significance of the association between CT and HPV infection remains to be elucidated.

Keyword

Cervical intraepithelial neoplasia; Human papilloma virus; Chlamydia trachomatis; Liquid-based cytology

MeSH Terms

Biopsy
Carcinoma in Situ
Cervical Intraepithelial Neoplasia
Chlamydia
Chlamydia trachomatis
Conization
DNA
Female
Human Papillomavirus DNA Tests
Humans
Polymerase Chain Reaction
Prevalence
DNA

Figure

• 

  Fig. 1 Species-specific DNA amplification by multiples polymerase chain reaction in clinical specimens. M: STD6 marker: Trichomonas vaginalis (580 bp), Mycoplasma hominis (502 bp), Ureaplasma urealyticum (435 bp), Chlamydia trachomatis (348 bp), Mycoplasma genitalium (253 bp), Neisseria gonorrhoeae (214 bp). N: negative. 1~6: clinical samples. Sample 1 Trichomonas vaginalis, Chlamydia trachomatis. Sample 2 Ureaplasma urealyticum. Sample 3 Mycoplasma hominis, Chlamydia trachomatis. Sample 4 Neisseria gonorrhoeae. Sample 5 Mycoplasma genitalium. Sample 6 Ureaplasma urealyticum, Chlamydia trachomatis, Mycoplasma genitalium, Neisseria gonorrhoeae.

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