Korean J Urol.  1992 Apr;33(2):206-212.

Expression of c-myc and c-ha-ras oncogenes in human bladder cancer

• Park CH
• Suh MH
• Lee SC

Abstract

In order to elucidate the c-myc and c-Ha-ras oncogenes expressions in human bladder cancers. we examined twelve bladder cancer tissues. two normal bladder tissues and two blood specimens with Southern. Northern and Slot blot hybridizations. In Southern blot all of the specimens showed 14 kilobase(Kb) c-myc band and 6.6kb c-Ha-ras band. No significant amplifications were observed in the levels of c-myc and c-Ha-ras DNA in cancer tissues. In Northern and slot blot, four bladder cancer tissues showed increased expression of c-myc RNA. No significant differences were observed in the levels of c-Ha-ras RNA expression between cancer tissues and normals. In conclusion. these findings support that quantitative increase in c-myc expression plays its role in the development of bladder cancers. Further studies about c-Ha-ras point mutation analysis and P21 ras protein analysis should be done to identify the role or c-Ha-ras in bladder tumorigenesis.

Keyword

Bladder cancer; C-myc; C-Ha-ras; Oncogene

MeSH Terms

Blotting, Southern
Carcinogenesis
DNA
Humans*
Oncogenes*
Point Mutation
RNA
Urinary Bladder Neoplasms*
Urinary Bladder*
DNA
RNA