Korean J Anat.  2005 Jun;38(3):285-293.

Comparison of the Effects of NOS Inhibitor or HS-1580 on Nigrostriatal Dopaminergic System in MPTP-induced Parkinson's Disease Animal Model

• Baek SY
• Park IS
• Oh SO
• Choi WC

Abstract

The antioxidant effects of HS-1580 derived from Brown seaweeds in comparison with NOS inhibitor, L-NAME, were tested in the Parkinson's disease animal model. C57BL/6 mice were implanted with osmotic pump containing vehicle, HS-1580 or L-NAME intraperitoneally 2 days before MPTP injection. The Parkinson's animal model were prepared by intraperitonal injection of MPTP (20 mg/kg, 4 times with 2 hours intervals in a ay).The mice were perfused with Zamboni fixative 2 days or 7days after MPTP injection. The 30 micrometer cryostat section were immunostained with free-floating method. Rabbit anti-tyrosine hydroxylase (TH)and rat anti-mouse MAC-1 were used as the primary antibodies. The following results were obtained. The number of TH-immunoreactive (ir)neurons in substantia nigra (SN)and the relative density of TH-ir axon terminals in striatum were decreased by MPTP injection. But the ecrease was significantly attenuated with 10%HS-1580 or L-NAME (50 mg/kg)pretreatment before MPTP injection. MAC-1-ir activated microglia were observed in substantia nigra 2 days after MPTP injection. Activated microglia were showed as thickened processes with round cell bodies. The morphological changes of MAC-1-ir activated microglia were inhibited by HS-15980 or L-NAME pretreatment before MPTP injction. Above results mean that the damage of nigrostriatal dopaminergic system was rescued by pretreatment of HS-1580 or L-NAME in MPTP-induced Parkinson's disease animal model.

Keyword

HS-1580; L-NAME; MPTP

MeSH Terms

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Animals*
Antibodies
Antioxidants
Mice
Microglia
Models, Animal*
NG-Nitroarginine Methyl Ester
Parkinson Disease*
Presynaptic Terminals
Rats
Specific Gravity
Substantia Nigra
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Antibodies
Antioxidants
NG-Nitroarginine Methyl Ester