Korean J Anat.  2005 Jun;38(3):235-242.

Comparative Gene Analyses between Benign Prostatic Hyperplasia and Cancer Using CGH

• Kim HJ
• Kim SH
• Kim SG
• Kim BY

Abstract

The present study was performed to find genetic changes in both cancer and hyperplastic cells in the prostate gland, elucidating any plausible interrelations of the changes between them. Nine cases of microdissected adenocarcinoma cells and 10 cases of microdissected hyperplastic cells in prostate tumors were analyzed to produce a map of DNA copy number changes throughout the entire genome using DOP-PCR and comparative genomic hybridization. The chromosome number of showing copy number changes was more in cancer cells than in hyperplastic cells. Chromosome regions Xq and 8q, where near 8q21 region was highly amplified, were the most frequent sites of gain in both cancer and hyperplastic cells. The most common region of loss in cancer cells was a chromosome region 8p (56%in frequency), followed by 16q.On the contrary, any high frequency of deletion could not be found in hyperplastic cells, contrary to the deletion in cancer cells. It is, therefore suggested that chromosome 8p deletion is a key factor toward progressing into adenocarcinoma, and that oncogene (s)near 8q 21 might contribute to a common pathway to progress into both hyperplastic or precancerous and cancer cells in prostate tumor.

Keyword

CGH; Hyperplasia; Cancer; Prostate

MeSH Terms

Adenocarcinoma
Comparative Genomic Hybridization
DNA Copy Number Variations
Genome
Hyperplasia
Oncogenes
Prostate
Prostatic Hyperplasia*