Effects of Chromium Exposure on the Level of Urinary 8-Hydroxydeoxyguanosine

Kim, Seong Joon; Yu, Byung Chul; Ohm, Sang Hwa; Jeong, Ki Won; Sung, Luck Hee; Shin, Won Chang; Cho, Jong Rae; Chun, Jin Ho

Abstract

OBJECTIVES
To investigate the possibility of utilizing DNA adduct as a carcinogenic biological marker for workers exposed to chromium, and the effect of chromium exposure on the formation of urinary 8-hydroxydeoxyguanosine(8-OH-dG) was also evaluated.
METHODS
The chromium concentrations of venous blood and urine were measured in 20 chromium exposed workers(exposure group) and in 11 chromium workers(control group) who were not exposed. The concentration of 8-OH-dG in their urine was determined using high performance liquid chromatography with electrochemical detection.
RESULTS
The blood chromium concentration was significantly higher in the exposure group ( 0.46+/-0.18 microgram/100 ml) than in control group(0.27+/-0.15 microgram/100 ml), but the urinary chromium concentration was not significantly higher in the exposure group. The urinary 8-OH-dG was higher in the exposure group(1.71+/-1.82 micromol/mol creatinine) than that in the control group(0.45+/-0.46 micromol/mol creatinine) and was significantly correlated with the blood chromium concentration(r=0.49). Results of multiple regression analysis revealed that the level of urinary 8-OH-dG depended upon the level of the blood chromium conc entration ( r2= 0.21).
CONCLUSIONS
Urinary 8-OH-dG was significantly related to chromium exposure and this finding suggests the possibility that urinary 8-OH-dG could be used as a biological index of chromium induced DNA damage.