J Korean Radiol Soc.  2007 Feb;56(2):203-208. 10.3348/jkrs.2007.56.2.203.

Relationship between the Number of SPIO-labeled Macrophage and MR Signal Intensity

• Lee HJ
• Lee JS
• Do KH
• Kang J
• Jang SS

• KMID: 2024503
• DOI: http://doi.org/10.3348/jkrs.2007.56.2.203

Abstract

PURPOSE
For the in vivo imaging of macrophages using MRI, the feasibility of labeling macrophages with iron oxide and the number of SPIO-labeled macrophage detected in 1.5 T MR, were assessed.
MATERIALS AND METHODS
The MR signal intensity was measured with variable concentrations of iron oxide, ranging from 112 to 2.384x10-7 μgFe/mL. The macrophages were incubated in SPIO solution (112 μgFe/mL) for 24 hours. The MR signal intensity was measured in variable numbers of SPIO-labeled macrophages.
RESULTS
The MR image signal intensity gradually increased with decreasing SPIO concentration, and reached a plateau at a concentration of 0.219 μgFe/mL. After incubation with iron oxide, the compact uptake of SPIO was detected in the cytoplasm of the macrophages using Prussian blue staining. No susceptibility effect was detected in the tubes of more than 122 macrophages.
CONCLUSION
The MR signal intensity was dependent on the number of macrophages. No susceptibility effect due to a cluster of SPIO-labeled macrophages was detected in more than 488 cells.

Keyword

Phantoms; Magnetic resonance (MR), experimental studies; Magnetic resonance (MR), contrast enhancement

MeSH Terms

Cytoplasm
Iron
Macrophages*
Magnetic Resonance Imaging
Iron