Korean J Obstet Gynecol.  2003 Jan;46(1):28-33.

Comparative Analysis of Multiplex PCR and Hybrid Capture System in the Detecttion of Human Papillomavirus in the Uterine Cervix

• Han SJ
• Kim S
• Kang MC
• Lim JS
• Ahn TG
• Lee BR

Abstract

OBJECTIVE
To compare the multiplex-PCR and hybrid capture methods for detection of Human papillomavirus (HPV) in uterine cervical swab samples.
METHODS
This study determined the HPV infection and its risk group (subtype) in uterine cervical swab samples of 91 Korean women by HPV hybrid capture I and multiplex-PCR method.
RESULTS
The prevalence of HPV infection determined by muliplex-PCR was more higher than by hybrid capture method I. Of 65 women with nonspecific uterine cervical lesions, the frequence of HPV-positivity was 14/65 (12 low-risk and 2 high-risk HPV) by hybrid capture method I and was 37/65 (34 low-risk and 3 high- risk HPV) by multiplex-PCR method, respectively. The frequence of high-risk HPV was 2/6 in LSIL, 7/14 in HSIL, 4/6 in uterine cervical cancer by hybrid capture method I, and was 2/6 in LSIL, 8/14 in HSIL, 4/6 in uterine cervical cancer by multiplex-PCR method.
CONCLUSION
These results suggest that multiplex-PCR is more sensitive, simple and cost-effective technique than Hybrid-capture I in the detection of HPV infection in the uterine cervix.

Keyword

HPV; Multiplex PCR; Hybrid capture I

MeSH Terms

Cervix Uteri*
Female
Humans*
Multiplex Polymerase Chain Reaction*
Prevalence
Uterine Cervical Neoplasms