Ann Lab Med.  2012 May;32(3):201-205. 10.3343/alm.2012.32.3.201.

Comparison of the AdvanSure Human Papillomavirus Screening Real-Time PCR, the Abbott RealTime High Risk Human Papillomavirus Test, and the Hybrid Capture Human Papillomavirus DNA Test for the Detection of Human Papillomavirus

• Hwang Y
• Lee M

• KMID: 1381685
• DOI: http://doi.org/10.3343/alm.2012.32.3.201

Abstract

BACKGROUND
We evaluated the performance of various commercial assays for the molecular detection of human papillomavirus (HPV); the recently developed AdvanSure HPV Screening real-time PCR assay (AdvanSure PCR) and the Abbott RealTime High Risk HPV PCR assay (Abbott PCR) were compared with the Hybrid Capture 2 HPV DNA Test (HC2).
METHODS
All 3 tests were performed on 177 samples, and any sample that showed a discrepancy in any of the 3 tests was genotyped using INNO-LiPA HPV genotyping and/or sequencing. On the basis of these results, we obtained a consensus HPV result, and the performance of each test was evaluated. We also evaluated high-risk HPV 16/18 detection by using the 2 real-time PCR assays.
RESULTS
Among the 177 samples, 65 were negative and 75 were positive in all 3 assays; however, the results of the 3 assays with 37 samples were discrepant. Compared with the consensus HPV result, the sensitivities and specificities of HC2, AdvanSure PCR, and Abbott PCR were 97.6%, 91.7%, and 86.9% and 83.9%, 98.8%, and 100.0%, respectively. For HPV type 16/18 detection, the concordance rate between the AdvanSure PCR and Abbott PCR assays was 98.3%; however, 3 samples were discrepant (positive in AdvanSure PCR and negative in Abbott PCR) and were confirmed as HPV type 16 by INNO-LiPA genotyping and/or sequencing.
CONCLUSIONS
For HPV detection, the AdvanSure HPV Screening real-time PCR assay and the Abbott PCR assay are less sensitive but more specific than the HC2 assay, but can simultaneously differentiate type 16/18 HPV from other types.

Keyword

Human papillomavirus; Real-time PCR; HPV type 16

MeSH Terms

Adult
Aged
Cervix Uteri/pathology/virology
DNA, Viral/analysis
Female
Genotype
Human papillomavirus 16/genetics
Human papillomavirus 18/genetics
Humans
Middle Aged
Papillomaviridae/*genetics/isolation & purification
Papillomavirus Infections/*diagnosis/pathology/virology
Reagent Kits, Diagnostic
Sensitivity and Specificity
Young Adult