Exp Neurobiol.  2014 Mar;23(1):77-85. 10.5607/en.2014.23.1.77.

Mesenchymal Stem Cells Improved the Ultrastructural Morphology of Cerebral Tissues after Subarachnoid Hemorrhage in Rats

Affiliations
  • 1Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. fsadeghian@gmail.com
  • 2Stem Cell Biology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
  • 3Yazd ACECR Higher Education Institute, Yazd, Iran.
  • 4Department of Biology, Faculty of Science, Sistan & Baluchestan University, Zahedan, Iran.
  • 5Department of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Abstract

Subarachnoid hemorrhage (SAH) causes widespread disruption in the cerebral architecture.The process of SAH is complicated and many people lose their lives or become disabled after injury. Mesenchymal stem cells (MSCs) are considered as good candidate for repair of cerebral damage. The aim was to assess the ultrastructural changes in the rat cerebral tissue after intravenous transplantation of MSCs. Female Wistar rats (8 per group) weighing 275~300 g were assigned to control (SAH+PBS) and experimental groups (SAH+MSCs).The samples from middle cerebral arterial wall and parietal cerebral tissue were prepared for transmission electron microscopy (TEM) according to standard protocol. Fine architectures of the vessel wall, including the contraction of the inner layer, smooth muscle layer,as well as neural cells were observed after SAH. Cerebral arterial wall and cortex, including neuronal and glial cells were injured post SAH. But, administration of MSCs improved the structural integrity of cerebral tissues. Changes were much more balanced with their relative improvement in some areas. The role of MSCs for repairing the injured cerebral tissues post experimental SAH was approved by electron microscopy.

Keyword

Electron microscopy; Mesenchymal stem cell; subarachnoid hemorrhage; Transplantation; rat

MeSH Terms

Animals
Female
Humans
Mesenchymal Stromal Cells*
Microscopy, Electron
Microscopy, Electron, Transmission
Muscle, Smooth
Neuroglia
Neurons
Rabeprazole
Rats*
Rats, Wistar
Subarachnoid Hemorrhage*
Transplantation
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