J Korean Soc Transplant.  2006 Jun;20(1):63-68.

Detection of Donor Specific Anti-HLA Antibodies Using Antibody Monitoring System

• Oh EJ
• Park YJ
• Kim JY
• Yang CW
• Kim DG
• Moon IS

Abstract

PURPOSE: The antibody monitoring system (AMS, GTI Inc.) is a solid phase ELISA crossmatch test for the detection of IgG antibody to the donor-specific solubilized HLA class I and class II antigens. The objective of this study was to compare the results of AMS assay with donor specific anti-HLA IgG antibodies (DS-HLA Abs), as determined by ELISA-PRA and flowcytometric crossmatch test (FCXM).
METHODS
A total of 132 sera were tested for the presence of DS-HLA Abs by ELISA-EIA, FCXM and AMS assay.
RESULTS
DS-HLA Abs were determined in 41 serum samples by an ELISA-PRA panel and FCXM. There was a significant degree of concordance (84.8%) between the results from the FCXM and AMS (P<0.001). The sensitivity, specificity, the positive predictive value and the negative predictive value of AMS assay to detect DS-HLA Abs was 90.2%, 93.4%, 86.0%, 95.5%, respectively. The AMS is a simple, objective test and it has several advantages over the cell-based crossmatch test such as elimination of non-HLA antibody reactivity, elimination of the non-donor specific antibody reactivity, no need for viable cells, and the donor's HLA antigens can be prepared in advance.
CONCLUSION
This study suggests that AMS may be useful as a supportive crossmatch test or as a monitoring test after transplantation for detecting class I and/or class II DS-HLA Abs.

Keyword

Transplantation; Donor-specific HLA antibody; Panel reactive antibody (PRA); Flowcytometric crossmatch; Antibody monitoring system (AMS)

MeSH Terms

Antibodies*
Enzyme-Linked Immunosorbent Assay
Histocompatibility Antigens Class II
HLA Antigens
Humans
Immunoglobulin G
Sensitivity and Specificity
Tissue Donors*
Transplantation
Antibodies
HLA Antigens
Histocompatibility Antigens Class II
Immunoglobulin G