Treatment of Diabetic Mouse Wounds using Blood Bank Platelet Concentrates

Han, Seung Kyu; Jeong, Seong Ho; Lee, Byung Il; Kim, Woo Kyung; Gottrup, Finn

Abstract

PURPOSE: Many clinical trials have shown the effectiveness of platelet releasate on chronic wounds. However, a large volume of blood must be aspirated from a patient and a platelet separator is required. Here, we hypothesized that platelet concentrate obtained from a blood bank (PCBB) would be also effective at stimulating wound healing. The purpose of this study was to investigate the effectiveness of PCBB on accelerating healing of diabetic wounds in vivo.
METHODS
Round wounds of 5mm diameter were made at four sites(two wounds on the left and two on the right side) on the backs of nine diabetic mice. Three hundred million platelets suspended in 0.05ml fibrinogen were dispersed on each wound on left sides. Same amount of fibrinogen without platelets was dispersed on right side control wounds. Thereafter, 0.05ml thrombin was applied to the each wound. Ten days after wound treatment, healed wounds were excised and the extent of wound healing in each group was compared.
RESULTS
Quantitative histologic analysis of epithelial gap distances revealed that PCBB treatment had greatly accelerated wound healing. Mean epithelial gap distances for PCBB treated and control wounds were 2.5x0.6mm and 3.6x0.5mm, respectively(p<0.05).
CONCLUSION
Our results suggest that PCBB has potential to accelerate the healing of diabetic wounds.