J Korean Acad Prosthodont.  2013 Oct;51(4):245-251. 10.4047/jkap.2013.51.4.245.

Comparison of biofilm on titanium and zirconia surfaces: in vivo study

• Lim KT
• Lee JH
• Lim IG
• Park SH
• Lim HP
• Kim OS

• KMID: 2000147
• DOI: http://doi.org/10.4047/jkap.2013.51.4.245

Abstract

PURPOSE
This study was conducted to compare in vivo biofilm formation on titanium surface and zirconia surface.
MATERIALS AND METHODS
For biofilm formation on titanium and zirconia in oral cavity, after producing oral appliances using acrylic resin and orthodontic wire tailored to 9 subjects, we made titanium and zirconia specimens (6 mm x 6 mm x 2 mm), fixed them on oral appliances and maintained them in oral cavity of test subjects for 24 and 72 hours. Test subjects who have equipped two pairs of specimens maintained oral hygiene not by using toothpaste but only by tooth brushing. After 24 and 72 hours, we removed and observed specimens through scanning electron microscopy (SEM).
RESULTS
Biofilm formation showed large deviation depending on individuals. For formation comparison between titanium and zirconia for 24 hours, zirconia showed less biofilm formation than titanium. Biofilm formation showed large deviation depending on individuals. As for formation comparison between zirconia and titanium, the degree of biofilm formation in zirconia was less than it was in titanium after a lapse of 24 hours. The result of biofilm formation in 72 hours trial show that zirconia has an inclination to formate less biofilm than it was in titanium.
CONCLUSION
Based on the above results, we can conclude that early biofilm formation in oral cavity was influenced by difference of abutment materials.

Keyword

Titanium surface; Zirconia surface; Biofilm

MeSH Terms

Biofilms*
Microscopy, Electron, Scanning
Mouth
Oral Hygiene
Orthodontic Wires
Titanium*
Tooth
Toothpastes
Zirconium
Titanium
Toothpastes
Zirconium

Figure

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  Fig. 1. Experimental appliance model.

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  Fig. 2. Bacterial adhesion on titanium and zirconia Surfaces (magnification ×50). A: 24 hrs titanium surface, B: 24 hrs zirconia surface, C: 72 hrs titanium surface, D: 72 hrs zirconia surface.

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  Fig. 3. Plaque composition (Rod %) attachment to titanium and zirconia surfaces after 24 hrs and 72 hrs (magnification ×10,000, 12 × 10 ㎛ size specimen). A: 24 hrs zirconia, B: 24 hrs titanium, C: 72 hrs zirconia, D: 72 hrs titanium.

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