J Korean Acad Periodontol.  2008 Dec;38(4):645-656. 10.5051/jkape.2008.38.4.645.

Effects of H2O2 and chlorhexidine on MMP-1, TIMP-1,2, Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts

• Choi SM
• Jang HS
• Kim BO

• KMID: 1999597
• DOI: http://doi.org/10.5051/jkape.2008.38.4.645

Abstract

PURPOSE
To evaulate the effects of chlorhexidine and H2O2 on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase(TIMP-1, TIMP-2), Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts (hPDLF).
MATERIALS AND METHODS
1.2x10(-1)%, 1.2x10(-2)% and 1.2x10(-3)% CHX and 3x10(-3)%, 3x10(-4)% and 3x10(-5)% H2O2 and mixture of CHX and H2O2 were applied to hPDLF for 1 min and 30 min. The mRNA expressions of MMP-1, TIMP-1 and 2, Type 1 collagen, fibronectin and UNCL in hPDLF were analysed by RT-PCR.
RESULTS
The result were as follows: 1. The expression of UNCL mRNA was higher than that of other mRNAs. 2. 1.2x10(-3) % CHX increased mRNA expressions of hPDLF as application time increased. 3. H2O2 lower than 3x10(-3) % increased expression of UNCL mRNA, and did not decrease mRNA expression of hPDLF. 4. hPDLF treatment with 1.2x10(-1) % CHX (with or without H2O2) resulted in no gene expression. 5. hPDLF treatment with 1.2x10(-2) % CHX (with or without H2O2) for 30 minutes resulted in no gene expression.
CONCLUSION
Because low concentration of CHX and H2O2 increased UNCL mRNA expression of hPDLF, low concentraction of CHX and H2O2 may have an antioxidative effect.

Keyword

chlorhexidine; H2O2; human periodontal ligament fibroblasts

MeSH Terms

Chlorhexidine
Collagen Type I
Fibroblasts
Fibronectins
Humans
Matrix Metalloproteinase 1
Periodontal Ligament
RNA, Messenger
Tissue Inhibitor of Metalloproteinase-1
Chlorhexidine
Collagen Type I
Fibronectins
Matrix Metalloproteinase 1
RNA, Messenger
Tissue Inhibitor of Metalloproteinase-1