J Korean Acad Conserv Dent.  2008 Mar;33(2):141-147. 10.5395/JKACD.2008.33.2.141.

Development of nano-fluid movement measuring device and its application to hydrodynamic analysis of dentinal fluid

Affiliations
  • 1Department of Conservative Dentistry, School of Dentistry, Seoul National University, Korea. inboglee@snu.ac.kr

Abstract

This study was aimed to develop an instrument for real-time measurement of fluid conductance and to investigate the hydrodynamics of dentinal fluid. The instrument consisted of three parts; (1) a glass capillary and a photo sensor for detection of fluid movement, (2) a servo-motor, a lead screw and a ball nut for tracking of fluid movement, (3) a rotary encoder and software for data processing. To observe the blocking effect of dentinal fluid movement, oxalate gel and self-etch adhesive agent were used. BisBlock (Bisco) and Clearfil SE Bond (Kuraray) were applied to the occlusal dentin surface of extracted human teeth. Using this new device, the fluid movement was measured and compared between before and after each agent was applied. The instrument was able to measure dentinal fluid movement with a high resolution (0.196 nL) and the flow occurred with a rate of 0.84 to 15.2 nL/s before treatment. After BisBlock or Clearfil SE Bond was used, the fluid movement was decreased by 39.8 to 89.6%.

Keyword

Dentinal tubule; Water conductance; Dentin hypersensitivity; Oxalate; Self etching adhesive; Hydrodynamics

MeSH Terms

Adhesives
Capillaries
Dentin
Dentin Sensitivity
Dentinal Fluid
Glass
Humans
Hydrodynamics
Nuts
Oxalic Acid
Resin Cements
Tooth
Track and Field
Adhesives
Oxalic Acid
Resin Cements

Figure

  • Figure 1-a Schematic diagram of the nano-flow measurement device.

  • Figure 1-b Circuit diagram of photo sensor and servo amplifier.

  • Figure 2 Representative curves of dentinal fluid flow before and after application of BisBlock (a) or Clearfil SE bond (b).


Cited by  2 articles

Real-time measurement of dentinal tubular fluid flow during and after amalgam and composite restorations
Sun-Young Kim, Byeong-Hoon Cho, Seung-Ho Baek, Bum-Sun Lim, In-Bog Lee
J Korean Acad Conserv Dent. 2009;34(6):467-476.    doi: 10.5395/JKACD.2009.34.6.467.

Real-time measurement of dentinal fluid flow during desensitizing agent application
Sun-Young Kim, Eun-Joo Kim, In-Bog Lee
J Korean Acad Conserv Dent. 2010;35(5):313-320.    doi: 10.5395/JKACD.2010.35.5.313.


Reference

1. Roberson TM, Heymann HO, Swift EJ. Art and science of operative dentistry. 2002. 4th edi. Mosby;14–62.
2. Summitt JB, Robbins JW, Schwartz RS. Fundamentals of operative dentistry. 2001. 2nd edi. Quintessence publishing co, Inc;8–22.
3. Brännström M. Dentin and pulp in restorative dentistry. 1981. Wolfe medical.
4. Hirata K, Nakashima M, Sekine I, Mukouyama Y, Kimura K. Dentinal fluid movement associated with loading of restorations. J Dent Res. 1991. 70:975–978.
Article
5. Orchardson R, Cadden SW. An update on the physiology of the dentin-pulp complex. Dent Update. 2001. 28:200–209.
6. Brännstorm M, Lindén L, Johnson G. Movement of dentinal and pulpal fluid caused by clinical procedures. J Dent Res. 1968. 5:679–682.
Article
7. Pashley DH, Derkson GD, Tao L, Kalathoor S. The effect of a multi-step dentin bonding system on dentin permeability. Dent Mater. 1988. 4:60–63.
8. Sauro S, Pashley DH, Montanari , Chersoni S, Carvalho RM, Toledano M, Osorio R, Tay FR, Prati C. Effect of simulated pulpal pressure on dentin permeability and adhesion of self-etch adhesives. Dent Mater. 2007. 23:705–713.
Article
9. Paphangkorakit J, Osborn JW. The effect of normal occlusal forces on fluid movement through human dentin in vitro. Arch Oral Biol. 2000. 45:1033–1041.
Article
10. De La Macorra JC, Escribano NI. Comparison of two methods to measure permeability of dentin. J Biomed Mater Res. 2002. 63:531–534.
Article
11. Ozok AR, Wu MK, ten Cate JM, Wesselink PR. Effect of dentinal fluid composition on dentin demineralization in vitro. J Dent Res. 2004. 83:849–853.
Article
12. Gregoire G, Joniot S, Guignes P, Millas A. Dentin permeability: self etching and one bottle dentin systems. J Prosthet Dent. 2003. 90:42–49.
13. Pashley DH, Matthews WG, Zhang Y, Johnson M. Fluid shifts across human dentin in vitro in response to hydrodynamic stimuli. Arch Oral Biol. 1996. 41:1065–1072.
Article
14. Ciucchi B, Bouillaguet S, Holz J, Pashley D. Dentinal fluid dynamics in human teeth, In vivo. J Endod. 1995. 21:191–193.
15. Kolker JL, Vargas MA, Armstrong SR, Dawson DV. Effect of desensitizing agents on dentin permeability and dentin tubule occlusion. J Adhes Dent. 2002. 4:211–221.
16. Ratih DN, Palamara JEA, Messer HH. Dentinal fluid flow and cuspal displacement in response to resin composite restorative procedures. Dent Mater. 2007. 23:1405–1411.
Article
17. Ratih DN, Palamara JEA, Messer HH. Temperature change, dentinal fluid flow and cuspal displacement during resin composite restoration. J Oral Rehab. 2007. 34:693–701.
Article
18. Ciucchi B, Bouillaguet S, Delaloye M. Volume of the internal gap formed under composite restorations in vitro. J Dent. 1997. 25:305–312.
Article
19. Martin RL, Brackett WW, Loushine RJ, Rockman RA, Ferrari M, Pashley DH, Tay FR. Sealing properties of mineral trioxide aggregate orthograde apical plugs and root fillings in an in vitro apexification model. J Endod. 2007. 33:272–275.
Article
20. Beneson W, Harris JW, Stocken H, Lutz H. Handbook of physics. 2006. Springer;153–210.
21. Breschi L, Mazzoni A, Ruggeri A, Cadenaro M, Linarda RD, Dorigo EDS. Dental adhesion review: Aging and stability of bonded interface. Dent Mater. 2008. 24:90–101.
22. Tay FR, Pashley DH. Water treeing-a potential mechanism for degradation of dentin adhesives. Am J Dent. 2003. 16:6–12.
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