Korean J Physiol Pharmacol.  2000 Apr;4(2):99-104.

Differential effects of nitric oxide synthase inhibitors in rats

• Lee JH
• Shin CY
• Kang BS
• Jeong JH
• Choi KB
• Min YS
• Kim JH
• Huh IH
• Sohn UD

Abstract

We investigated the action of NOS inhibitors on NOS in rats. Both of nitric oxide synthase inhibitors, NG-monomethyl-L-arginine (L-NMMA, 3 micrometer) or NG-nitro-L-arginine methylester (L-NAME, 30 micrometer), augmented phenylephrine (PE, 10-7 M)-induced contraction which was inhibited by acetylcholine (ACh) in rat thoracic aorta. This augmentation by L-NAME or L-NMMA was attenuated with the treatment of NO precursor, arginine. ACh, however, decreased the augmentation induced by L-NMMA, but not by L-NAME. Superoxide dismutase (SOD, 50 u/ml) potentiated an inhibitory effect of ACh on the PE (10-7 M)-induced contraction. It has been known that platelet activating factor itself induces iNOS. Platelet activating factor (PAF, 10-7 M) inhibited PE (10-7 M)-induced contraction. Pretreatment with L-NMMA (30 mM) or L-NAME (30 mM) significantly blocked the inhibitory action of PAF on PE-induced contraction. L-NMMA (100 mM) or L-NAME (100 mM) reduced nerve conduction velocity (NCV) relevant to nNOS in rat sciatic nerve. ACh attenuated the reduction of NCV by L-NMMA-, but not by L-NAME-induced reduction of NCV. These results suggest that L-NMMA and/or L-NAME have different action on three types of NOS in rats.

MeSH Terms

Acetylcholine
Animals
Aorta, Thoracic
Arginine
Neural Conduction
NG-Nitroarginine Methyl Ester
Nitric Oxide Synthase*
Nitric Oxide*
Nitroarginine
omega-N-Methylarginine
Phenylephrine
Platelet Activating Factor
Rats*
Sciatic Nerve
Superoxide Dismutase
Acetylcholine
Arginine
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase
Nitroarginine
Phenylephrine
Platelet Activating Factor
Superoxide Dismutase
omega-N-Methylarginine