Clin Exp Otorhinolaryngol.  2014 Sep;7(3):210-215. 10.3342/ceo.2014.7.3.210.

The Promoting Effect of Carbamide Peroxide Teeth Bleaching Gel in a Preclinical Model of Head and Neck Cancer in Hamster Buccal Pouch

Affiliations
  • 1Laboratory of Medical Skills and Surgical Research, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil. vfbampi@gmail.com
  • 2Department of Food Sciences, Federal University of Lavras, Lavras, Brazil.
  • 3Department of Morphology Science, Federal University of Pelotas, Pelotas, Brazil.
  • 4Department of Surgery, Faculty of Medicine, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil.

Abstract


OBJECTIVES
The aim of this study was to verify the promoting effect of carbamide peroxide on dimethylbenzanthracene (DMBA)-induced carcinogenesis in the hamster buccal pouch, in order to reduce the period of latency for tumor formation.
METHODS
Sixteen hamsters were randomized into two groups of eight animals each. The hamsters of the group I had their right buccal pouches treated with 0.5% DMBA and 10% carbamide peroxide teeth bleaching gel for 55 days. The animals of the group II had their right pouches treated only with DMBA. After, six animals of each group had their pouches prepared for light microscopy. Histomorphometry was performed to assess the presence of keratinization, nuclear polymorphism, pattern of invasion, number of blood vessels, and inflammatory infiltrate in the tumor front. Furthermore, the newly formed lesions were graded according the Bryne's grading system. The remaining animals had the vascular system of the pouches casted by Mercox and qualitatively analyzed by scanning electron microscopy.
RESULTS
Histopathological analysis of the buccal pouches treated with DMBA and carbamide peroxide exhibited formation of squamous cell carcinoma well-differentiated with a high degree of malignancy in all pouches. The development of this neoplasm was associated with a significant increase in the number of blood vessels, presence of keratin pearls, and inflammatory infiltrate. The pouches of the group II showed inflammation, epithelial hyperplasia, dysplasia, and squamous cell carcinoma in only three right pouches. The analysis of the electron micrographs of the pouches chemically inducted with DBMA and carbamide peroxide reveled formation of a new vascular network characteristic of squamous cell carcinoma.
CONCLUSION
The protocol presented here, using DMBA associated with carbamide peroxide, shortens the period of latency to produce squamous cell carcinoma in the hamster buccal pouch, decreasing the time and costs of the experiments.

Keyword

Carbamide peroxide; Dimethylbenzanthracene; Carcinogens; Hamster

MeSH Terms

9,10-Dimethyl-1,2-benzanthracene
Animals
Blood Vessels
Carcinogenesis
Carcinogens
Carcinoma, Squamous Cell
Cricetinae*
Head and Neck Neoplasms*
Hyperplasia
Inflammation
Microscopy
Microscopy, Electron, Scanning
Tooth Bleaching*
Urea*
9,10-Dimethyl-1,2-benzanthracene
Carcinogens
Urea

Figure

  • Fig. 1 (A) Image of an everted left buccal pouch. (B) Image of a buccal pouch showing an exophytic tumor after 55 days of tumor induction with dimethylbenzanthracene and carbamide peroxide.

  • Fig. 2 (A, B) The control pouches presented a stratified squamous orthokeratinized epithelium followed by dense connective tissue (lamina propria), striated muscle tissue and loose connective tissue. (C, D) All the right buccal pouches of the group I presented squamous cell carcinoma with a high pattern of invasion, keratin pearls and an increased vascularity. (E, F) After 55 days of exposure to dimethylbenzanthracene exclusively, there was an increase in the thickness of keratin and epithelium layers, inflammation and dysplasia (light microscopy, ×10 in the right column and ×20 in the left column, H&E stain).

  • Fig. 3 Scanning electron micrographs of vascular casts of hamster buccal pouches. (A) Left buccal pouch of group I (×100). (B) Left buccal pouch of group II (×400). (C, D) Right buccal pouch of group I (×100, ×200). (E, F) Right buccal pouch of group II (×100, ×200). Main arteries and veins are colored in red and blue consecutively.


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