Cancer Res Treat.  2002 Feb;34(1):52-57.

Expression of Cell Surface Receptors on Human Glioblastoma Xenograft Model in NOD/SCID Mouse

• Oh KS
• Kim KU
• Park NH
• Seo SY
• Choi SS
• Huh GY

Abstract

PURPOSE: To obtain basic data for development of a glioblastoma-specific immunotoxin, the expression of variable cell surface receptors on a human glioblastoma xenograft model was evaluated, using NOD/SCID mice.
MATERIALS AND METHODS
We developed a xenograft model in NOD/SCID mice implanted with a human glioblastoma cell line (U-87MG). Immunohistochemical studies were performed on implanted tumor nodules (n=8) using antibodies against CD71, EGFR, IGF-IRalpha, CXCR4 and IL-4Ralpha.
RESULTS
Expression of IL-4Ralpha, in implanted tumornodules, was the highest of the cell surface receptors evaluated in this study. However, the endothelial cells in, and around, the tumor nodules also revealed immunopositivity against IL-4Ralpha. The immunoreactivity of IL-4Ralpha, and other surface receptors such as CD71, IGF-IRalpha and EGFR, was prominent in tumor nodules associated with tumor necrosis.
CONCLUSION
IL-4Ralpha would be a possible target for the development of glioblastoma-specific immunotoxin, although there are limitations due to its endothelial expression.

Keyword

Glioblastoma; Interleukin-4 receptor; SCID mouse

MeSH Terms

Animals
Antibodies
Cell Line
Endothelial Cells
Glioblastoma*
Heterografts*
Humans*
Immunotoxins
Mice*
Mice, SCID
Necrosis
Receptors, Cell Surface*
Antibodies
Immunotoxins
Receptors, Cell Surface