Ewha Med J.  2006 Sep;29(2):81-88. 10.12771/emj.2006.29.2.81.

Analysis of Circulating B-1 B Lymphocyte Subsets in Patients with Systemic Lupus Erythematosus

• Baik SJ
• Lee J
• Lee YH

• KMID: 1964005
• DOI: http://doi.org/10.12771/emj.2006.29.2.81

Abstract

OBJECTIVES
To define the abnormalities in homeostasis of B-1 B lymphocytes compartments in human SLE.
METHODS
Perpheral blood was obtained from 7 patients with untreated active SLE patients and same patients at the time of incative disease after immunosuppressive therapy. The frequencies of CD5+CD45RA(int) B-1a B lymphocyte, and CD5+CD45RA(low) B-1b B lymphocyte, and CD5-CD45RA(high) conventional B-2 lymphocyte subsets were analyzed. For the control group, peripheral blood from 7 healthy adults and 7 patients with infectious fever were utilized.
RESULTS
B-1a B lymphocyte subset was found at high frequency in active SLE patients compared to the fever control(33.5+/-15.0% vs 20.1+/-5.3%,p=0.01). In contrast, B-2 B lymphocyte subset was found at lower frequency compared to the fever control (65.6+/-15.1% vs 77.9+/-5.6%,p=0.04). No difference in frequency of B-1b B lymphocyte subsets was found between active SLE and the control. After immunosuppressive theray, B-1b B lymphocyte subset was markedly decreased with increase in B-2 B lymphocyte subset(p=0.05).
CONCLUSION
These results indicate that there are abnormalities in B cell conpartments with expansion of B-1a B lymphocyte subset and contraction of B-2 B lymphocyte subset associated with the disease activity in patients wite SLE.

Keyword

Systemic lupus erythematosus; B-Lymphocyte subsets

MeSH Terms

Adult
B-Lymphocyte Subsets*
B-Lymphocytes
Fever
Homeostasis
Humans
Lupus Erythematosus, Systemic*
Lymphocyte Subsets
Lymphocytes