J Bacteriol Virol.  2004 Sep;34(3):201-212.

Partial Nucleotide Sequence of Porcine Parvovirus (VRI-1 Strain): Identification of the Putative Defective Genomes

• Kim BH
• An SH
• Kweon CH
• Song JY
• Park JH
• Hyun BH
• Yang DK
• Tak RB

Abstract

A porcine parvovirus, designated as VRI-1, was isolated from a 30-day-old piglet. Replicative form of viral DNA from ST cells infected with VRI-1 was directly cloned into pUC19. The cloned DNA fragment contained the entire nonstructural and structural protein genes, covering approximately 85% of the viral genome. The nucleotide sequence of VRI-1 showed 99.4~99.5% identity in the nonstructural protein (NS) and 99.0~99.2% identity in the structural protein with previously reported PPV strains, respectively. Among the cloned genes, two types of defective genomes with deletion of 100 and 247 nucleotides at almost similar location of 3' region within NS gene were also identified in this study.

Keyword

Porcine parvovirus; VRI-1 strain; Nucleotide sequence

MeSH Terms

Base Sequence*
Clone Cells
DNA
DNA, Viral
Genome*
Genome, Viral
Nucleotides
Parvovirus, Porcine*
DNA
DNA, Viral
Nucleotides