J Bacteriol Virol.
2004 Sep;34(3):201-212.
Partial Nucleotide Sequence of Porcine Parvovirus (VRI-1 Strain): Identification of the Putative Defective Genomes
- Affiliations
-
- 1National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, Anyang 430-824, Korea. kimbh@nvrqs.go.kr
- 2College of Veterinary Medicine, Kyungpook National University Taegu 707-701, Korea.
Abstract
- A porcine parvovirus, designated as VRI-1, was isolated from a 30-day-old piglet. Replicative form of viral DNA from ST cells infected with VRI-1 was directly cloned into pUC19. The cloned DNA fragment contained the entire nonstructural and structural protein genes, covering approximately 85% of the viral genome. The nucleotide sequence of VRI-1 showed 99.4~99.5% identity in the nonstructural protein (NS) and 99.0~99.2% identity in the structural protein with previously reported PPV strains, respectively. Among the cloned genes, two types of defective genomes with deletion of 100 and 247 nucleotides at almost similar location of 3' region within NS gene were also identified in this study.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Complete Nucleotide Sequence of Genomic RNA of a Large-Plaque Forming Porcine Reproductive and Respiratory Syndrome Virus PL97-1/LP1
- Prevalence of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and porcine parvovirus from aborted fetuses and pigs with respiratory problems in Korea
- First detection and genetic characterization of porcine parvovirus 7 from Korean domestic pig farms
- Notable mutations of porcine parvovirus 1 and 4 circulating in commercial pig farms in South Korea
- Development of a novel vaccine against canine parvovirus infection with a clinical isolate of the type 2b strain
