The effect of human DBM(Grafton.) graft on skull defect in the rabbit

Kim, Jin Wook; Park, In Suk; Lee, Sang Han; Kim, Chin Soo; Jang, Hyun Jung; Kwon, Tae Geon; Kim, Hyun Soo

Abstract

In oral and maxillofacial surgery, bone graft is very important procedure for functional and esthetic reconstruction. So, many researcher studied about bone graft material like autogenous bone, allograft bone and artificial bone materials. The purpose of this study is to evaluate the quantity of bone generation induced by Grafton. graft, human allogenic demineralized bone matrix. Total 24 sites of artificial bony defects prepared using trephin bur(diameter 8 mm) on parietal bone of six adult New Zealand White rabbits. Experimental group had six defect sites which grafted Grafton.(0.1 cc). Active control group had nine defect sites, into which fresh autogenous bone harvested from own parietal bone was grafted and passive control group had nine defect sites without bone graft. After six weeks postoperatively, the rabbits were sacrificed. The defects and surrounding tissue were harvested and decalcified in 10% EDTA, 10% foamic-acid. Specimens were stained with HandE. New bone area percentage in whole defect area was measured by IMT(VT) image analysis program. Quantity of bone by Grafton. graft was smaller than that of autograft and larger than that of empty defects. In histologic view Grafton. graft site and autograft site showed similar healing progress but it was observed that newly formed bone in active control group was more mature. In empty defect, quantity and thickness of new bone formation was smaller than in Grafton.-grafted defect. Grafton. is supposed to be a useful bone graft material instead of autogenous bone if proper maintenance for graft material stability and enough healing time were obtained.